DNA オリゴ QC（品質管理）
GenCefe Biotechは、オリゴヌクレオチド製品向けに厳格な品質管理システムを確立しており、ISO9001およびISO13485認証を取得しています。標準的なQC項目に加え、科学研究や体外診断（IVD）などの異なる用途に対応した包括的なカスタマイズQC項目も提供しています。
オリゴ検出方法
| Detection Items (methods) | Equipment | Brief Introduction |
| Appearance | 30x magnifying glass inspection lamp | Detect the presence of undried product, impurities, etc. |
| Quantitative (UV) | UV spectrophotometer | Detect the quantitative of Oligos |
| Molecular weight (MS) | Electron Spray Mass Spectrometry | Detect the molecular weight of Oligos |
| Purity (HPLC) | High performance liquid chromatography | Detect the purity of Oligos |
| Purity (CE) | Capillary electrophoresis | |
| Purity (PAGE) | Vertical gel electrophoresis | |
| CT value(qPCR) | Fluorescent quantitative PCR | Detect the positive target detection capabilities of the qPCR primer & probes |
| Fluorescence value(qPCR) | Detect the fluorescence value of qPCR primer & probes when amplifying positive targets | |
Human genomic DNA contamination (qPCR) | Detection of human genomic DNA contamination in qPCR primer & probes. | |
| NTC amplification (qPCR) | Detect abnormal amplification signals of qPCR primer & probes in a template-free system. | |
| Cross Contamination Rate (NGS) | High throughput sequencer | Detect the probability of cross-contamination between different oligos |
オリゴQC項目
| Detection Item | Primer Type | Primer | qPCR Primer | qPCR Probe | NGS Primer | Note |
Property detection | Appearance | Dry powder: uniform powder or transparent solid, without obvious impurities | Standard Detection | |||
| Liquid: colorless/specific color liquid, clear and transparent, without obvious impurities | ||||||
Quantitative (UV) | Error rate(％)≤±10％ | |||||
Molecular weight (MS) | Error rate(％)s＋0.05％ | |||||
| Purity (HPLC) | RPC≥85%;MOP≥90%;PAGE≥95%;HPLC/HPLC IVD≥95% | Customiz Detection | ||||
| Purity (CE) | Vary according to the purification method and the oligo length | |||||
| Purity (PAGE) | Clear main product band, correct molecular weight, no obvious impurities | |||||
Function Detection | Ct Value (qPCR) | - | A normal amplification curve can be detected within 45 cycles, and the Ct value is within a reasonable range | Customize Detection | ||
Fluorescence value (qPCR) | - | According to the agreement | ||||
Contamination Detection | Human genomic DNA contamination (qPCR) | - | No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40 | |||
NTC amplification (qPCR) | - | No peaks should be detected in the reaction system within 40 cycles, or the Ct value ≥ 40 | ||||
Cross Contamination (NGS) | - | - | - | Cross Contamination Rate(%)≤0.1% | ||
