DNAオリゴ修飾
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GenCefeは、カスタマイズ可能なDNAオリゴの修飾・ラベリングサービスを提供しており、ビオチン、ジゴキシゲニン、リン酸、チオール、スルフヒドリル、アミノ、特殊塩基、各種蛍光基など100種類以上の修飾 に対応しています。納品される修飾プライマー／プローブは100%質量分析で検証済み で、精度と純度を保証しており、研究用途および産業グレードの修飾プライマー／プローブのニーズに対応可能です。
オリゴ修飾位置
DNAオリゴ修飾ケーススタディ
125 nt、スペーサー18修飾、多重ポリT配列、純度>95.58%、分子量バイアス<0.05%
DNA修飾基
| Rare Base | Fluorescence | Fluorescence | Intermediate | Others |
| dI dU m5C m6A 2' -O-Methyl base RNA base LNA | 5' Cy3 3' Cy3 5' Cy5 3' Cy5 5' FAM 3' FAM 5' HEX 5' TET 5'-FITC 3'-FITC 5' 6-JOE 3' 6-JOE 5' ROX 3' ROX 5' TAMRA 3' TAMRA 3' -HEX 3' -TET 5' Helix Flour555 3' Helix Flour555 5' Helix Flour575 3' Helix Flour575 5' Texas Red 3' Texas Red 5' Quasar 570 3' Quasar 570 5' Quasar 670 3' Quasar 670 | 5' Cy3.5 3' Cy3.5 5' Cy5.5NS 3' Cy5.5NS 5' Cy7NS 3' Cy7NS 5'&3' CAL Fluor Red 590 5'&3' CAL Fluor Red 610 5'&3' DABCYL 5'&3' AMCA 5'&3' DEAC 5'&3' MCA 5'-VIC 5'-SIMA | Int NH2 dT Int Biotin dT Int Cy3 dT Int Cy5 dT Int TAMRA dT Int ROX dT Int FAM dT Int HEX dT Int Dig dT Int CHCH dT Int DBCO dT Int Azide dT Int BHQ1 dT Int BHQ2 dT Int HS-SH C6 Int Alexa Flour dT Int Azobenzene Int Ferrocene dT dT- LC RED-610 dT- LC RED-640 dT- LC RED-705 dC-Fluorescein dC-Tamra dC-Amino dC-Rox dC-Digoxin dC-FITC dC-Texas Red | Azide DBCO Spacer9 Spacer(C12) Spacer C3 Spacer C18 dSpacer Inverted Base 5' -Carboxy C10 5' -Acridine 3' ddC Alexa Flour 647 Yakima Yellow Epoch Pyrene |
| Phosphorothioate | ||||
| S | ||||
| Phosphorylation | ||||
| 5' PHO 3' PHO | ||||
| Amino Linkers | ||||
| 5' NH2 C6 3' NH2 C7 5' NH2 C12 | ||||
| Thiol | Quenchers | |||
| 3' SH C3 5' SH C6 | 3' DABCYL 3' TAMRA 3' BHQ 1 3' BHQ 2 3' BHQ3 3' -MGB 3' eclipse | |||
| Biotin | ||||
| 5' Biotin 5' Biotin TEG 3' Biotin 3' Biotin TEG | ||||
| Digoxigenin | ||||
| 5' DIG 3' DIG |
両末端標識プローブ
| TAMRA | ECLIPS | BHQ1 | BHQ3 |
| 5' FAM-3' TAMRA 5' HEX-3' TAMRA 5' TET-3' TAMRA 5' JOE-3' TAMRA 5' VIC-3' TAMRA | 5' FAM-3' ECLIPS 5' HEX-3' ECLIPS 5' TAMRA-3' Eclipse 5' ROX-3' Eclipse 5' TET-3' Eclipse 5' JOE-3' Eclipse | 5' FAM-3' BHQ1 5' VIC-3' BHQ1 5' HEX-3' BHQ1 5' JOE-3' BHQ1 5' TET-3' BHQ-1 | 5' Quasar 670-3' BHQ3 5' CY5-3' BHQ3 |
| MGB | Dabcyl | BHQ2 | Others |
| 5' FAM-3' MGB 5' HEX-3' MGB 5' TET-3' MGB 5' JOE-3' MGB 5' ROX-3' MGB 5' Texas Red-3' MGB 5' Quasar 670-3' MGB 5' VIC-3' MGB | 5' FAM-3' DABCYL 5' HEX-3' DABCYL 5' TET-3' DABCYL 5' JOE-3' DABCYL 5' TAMRA-3' DABCYL 5' Texas Red-3' Dabcyl 5' ROX-3' Dabcyl 5' CY3-3' DABCYL 5' Cy5-3' DABCYL | 5' Cy5-3' BHQ2 5' Cy3-3' BHQ2 5' ROX-3' BHQ2 5' TET-3' BHQ2 5' JOE-3' BHQ2 5' HEX-3' BHQ2 5' TAMRA-3' BHQ2 5' Texas Red-3' BHQ2 5' Quasar 670-3' BHQ2 5' -FAM and 3' -BHQ2 | 5' Phos and 3' FAM 5' TAMRA and 3' Phos 5' C6-Biotin and 3' Biotin 5' C6-Biotin and 3' Cy5 5' C6-NH2 and 3' C3-Fam 5' C6-NH2 and 3' Cy3 5'Cy5 and SS 5' Cy5 and 3' C7-NH2 5' Cy5 and 3' SH 5' Dabcyl and 3' C3-Fam 5' DlG and 3' Digoxin 5' SH and 3' Cy3 5' Fam and 3' C3-Fam 5' TAMRA and 3' C3-Fam |
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